P-711 Are there any differences among blastocyst euploid and mosaicism rate (high or low) using fresh or frozen ejaculate sperm in donated oocytes?

Abstract Study question To compare euploid and mosaicism rate (high or low) among blastocysts derived from fresh frozen ejaculate sperm in donated oocytes. Summary answer No statistically significant differences were found on blastocyst between versus frozen. What is known already Even though 5%-10% autosomal aneuploidies 5%-100% sex chromosomes are paternally derived, the clinical relevance of paternal contribution to chromosome aneuploidy frequently overlooked. Besides, male infertility could contribute generation chromosomal abnormalities resulting embryos. Sperm cryopreservation a widely used technique assisted reproduction for different reasons. However, little regarding potential effect freezing embryo aneuploidies. design, size, duration Retrospective, observational multicenter study patients undergoing Preimplantation Genetic Testing Aneuploidies (PGT-A) January 2017 December 2022 IVIRMA Madrid, Valencia Barcelona. elucidate advance maternal age, 1,780 biopsied coming egg donation program analyzed. Autologous samples with less than 2 million spermatozoa per milliliter partners altered karyotype, abnormal fish any genetic disease excluded study. Participants/materials, setting, methods Embryos cultured regular incubators, an atmospheric concentration 5% O2, 6.5% CO2 temperature 37ºC. Blastocyst biopsy was performed either day 5 6 development analyzed through Next Generation Sequencing (NGS). done following manufacturer’s instructions (Sperm freeze, Origio). Continuous variables compared student´s t-test categorical Chi-square using SPSS statistical program. Main results role chance Mean age (44.3±5.20 years old vs 43.2±5.20 old), (46.67±33.05 mill/ml 35.1±32.92 mill/ml) not significantly group (p > 0.05). A total 1780 tested PGT-A included: 1267 (n = 194 patients) 513 81 patients). (64.5% 67.2%, p 0.278 ;) respectively. rate: High (>50% trophectoderm cells) low (30-50% comparable (2.7% 4.5%; 0.90) (3.9% 3.7%), Limitations, reasons caution This based retrospective data. Wider implications findings According our findings, there no sperm. Trial registration number Not Applicable